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Well, of there is a chance they are brother and sister, I personally wouldn't let that happen. I've read that many people do dad to daughter, mother to son sort of thing, but I've also read to never let a brother and sister breed, due to defects that can occur with the kids. With that knowledge alone, me personally wouldn't encourage it.

A couple things you can do with you not thinking she's pregnant, I would get a blood test done and send it to Biotracking. There is also a bleach test you can do, I'm not sure how accurate it is, as I've never done it. You could always Lute her, to throw her into heat, but then if you don't get the preggo test done to make sure, she will abort the kids.

My .2 cents, I'm no expert, but I have learned a lot being on here and doing my own research:)
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